Coding

Part:BBa_K5109013:Design

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-26)


DehaS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1


Design Notes

The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag. Those sites have been designed in order to extract the Hydrolase sequence from the surface display system in which it is inserted, and to be able to exchange it with other enzymes, without modifying the rest of the expression cassette.



Source

This part was identified with accurate bioinformatic research into Synechocystis PCC 6803 genome. The sequence of the part was then reported on Benchling and synthezised.

References